【英文标准名称】:StandardTestMethodforEnumerationofNon-TuberculosisMycobacteriainAqueousMetalworkingFluidsbyPlateCountMethod
【原文标准名称】:用板计数法计算水性金属加工液中非结核分枝杆菌的标准试验方法
【标准号】:ASTME2563-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E34.50
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:含水的;计算;流体;工业用油;润滑剂;金属加工;板;测试
【英文主题词】:acid-fastbacteria;hypersensitivitypneumonitis;metalworkingfluid;non-tuberculousMycobacteria;rapidlygrowingMycobacteria
【摘要】:Thismethodallowsfortherecoveryandenumerationofviableandculturable,non-tuberculosis,rapidlygrowingMycobacteria(M.immunogenum,M.chelonae,M.absessus,M.fortuitum,andM.smegmatis)inthepresenceofhighgramnegativebackgroundpopulationsinmetalworkingfluidfieldsamples.Duringthepastdecadeithasbecomeincreasinglyapparentthatnon-tuberculousMycobacteriaarecommonmembersoftheindigenousMWFbacterialpopulation.Thispopulationispredominantlycomprisedofgramnegativebacteriaandfungi.Mycobacterialcontaminationofmetalworkingfluidshasbeenputativelyassociatedwithhypersensitivitypneumonitis(HP)amongstmetalgrindingmachinists.Thedetectionandenumerationoftheseorganismswillaidinbetterunderstandingofoccupationalhealthrelatedproblemsandabetterassessmentofantimicrobialpesticideefficacy.Themeasurementofviableandculturablemycobacterialdensitiescombinedwiththetotalmycobacterialcounts(includingviableculturable(VC),viable-nonculturable(VNC)andnonviable(NV)counts)isusuallythefirststepinestablishinganypossiblerelationshipbetweenMycobacteriaandoccupationalhealthconcerns(forexample,HP).Themethodcanbeemployedinsurveystudiestocharacterizetheviable-culturablemycobacterialpopulationdensitiesofmetalworkingfluidfieldsamples.Thismethodisalsoapplicableforestablishingthemycobacterialresistanceofmetalworkingfluidformulationsbydeterminingmycobacteriumsurvivalbymeansofplatecounttechnique.ThismethodcanalsobeusedtoevaluatetherelativeefficacyofmicrobicidesagainstMycobacteriainmetalworkingfluids.1.1ThistestmethodcoversthedetectionandenumerationofviableandculturablerapidlygrowingMycobacteria(RGM),ornon-tuberculosisMycobacteria(NTM)inaqueousmetalworkingfluids(MWF)inthepresenceofhighnon-mycobacterialbackgroundpopulationusingstandardmicrobiologicalculturemethods.1.2Thedetectionlimitisonecolonyformingunit(CFU)/mLmetalworkingfluid.1.3ThistestmethodinvolvescultureoforganismsclassifiedasLevel2pathogens,andshouldbeundertakenbyatrainedmicrobiologistinanappropriatelyequippedfacility.ThemicrobiologistshouldalsobecapableofdistinguishingthediversecoloniesofMycobacteriafromothermicroorganismcoloniesonaPetridishandcapableofconfirmingMycobacteriabyacidfaststainingmethodThisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:E34
【国际标准分类号】:75_100
【页数】:3P.;A4
【正文语种】: